CST1 Antibody

ProSci
Product Code: PSI-22-321
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-321-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using CST1 Antibody (22-321) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using CST1 antibody (22-321) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human prostate using CST1 antibody (22-321) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse stomach using CST1 antibody (22-321) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CST1 Antibody (22-321) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CST1 antibody (22-321) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CST1 antibody (22-321) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using CST1 antibody (22-321) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CST1, Cystatin SN, Cystatin 1, Cystatin SA-I, Cystatin-1, Salivary cystatin-SA-1, Cystain-SA-I, Cystatin-SN
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-141 of human CST1 (NP_001889.2).
NCBI Gene ID #:
1469
NCBI Official Name:
cystatin SN
NCBI Official Symbol:
CST1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P01037
User NOte:
Optimal dilutions for each application to be determined by the researcher.