CST2 Antibody

ProSci
Product Code: PSI-22-322
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-322-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse heart, using CST2 antibody (22-322) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
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Immunofluorescence analysis of HeLa cells using CST2 antibody (22-322). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse heart, using CST2 antibody (22-322) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
Immunofluorescence analysis of HeLa cells using CST2 antibody (22-322). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
cystatin-SA, cystatin 2, cystatin S5, cysteine-proteinase inhibitor, salivary cysteine (thiol) protease inhibitor
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a secreted thiol protease inhibitor found at high levels in saliva, tears and seminal plasma.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-141 of human CST2 (NP_001313.1).
NCBI Gene ID #:
1470
NCBI Official Name:
cystatin SA
NCBI Official Symbol:
CST2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P09228
User NOte:
Optimal dilutions for each application to be determined by the researcher.