DARS Antibody

ProSci
Product Code: PSI-22-323
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-323-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using DARS Antibody (22-323) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using DARS antibody (22-323) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse spinal cord using DARS antibody (22-323) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse brain using DARS antibody (22-323) at dilution of 1:100 (40x lens).
5 / 5
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DARS antibody (22-323). Western blot was performed from the immunoprecipitate using DARS antibody (22-323) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using DARS Antibody (22-323) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using DARS antibody (22-323) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using DARS antibody (22-323) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DARS antibody (22-323) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DARS antibody (22-323). Western blot was performed from the immunoprecipitate using DARS antibody (22-323) at a dilition of 1:1000.

Further Information

Additional Names:
DARS, Aspartyl-tRNA synthetase, AspRS
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2).
NCBI Gene ID #:
1615
NCBI Official Name:
aspartyl-tRNA synthetase
NCBI Official Symbol:
DARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P14868
User NOte:
Optimal dilutions for each application to be determined by the researcher.