DDX1 Antibody

ProSci
Product Code: PSI-22-324
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-324-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 7
Western blot analysis of extracts of various cell lines, using DDX1 antibody (22-324) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 7
Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
5 / 7
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
6 / 7
Immunofluorescence analysis of MCF7 cells using DDX1 antibody (22-324). Blue: DAPI for nuclear staining.
7 / 7
Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug DDX1 antibody (22-324). Western blot was performed from the immunoprecipitate using DDX1 antibody (22-324) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using DDX1 antibody (22-324) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using DDX1 antibody (22-324). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug DDX1 antibody (22-324). Western blot was performed from the immunoprecipitate using DDX1 antibody (22-324) at a dilition of 1:1000.

Further Information

Additional Names:
DDX1, DBP-RB, UKVH5d
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100

IP: 1:20 - 1:50
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
NCBI Gene ID #:
1653
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box helicase 1
NCBI Official Symbol:
DDX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q92499
User NOte:
Optimal dilutions for each application to be determined by the researcher.