DDX41 Antibody

ProSci
Product Code: PSI-22-325
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-325-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DDX41 antibody (22-325) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat kidney using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat testis using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using DDX41 antibody (22-325). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DDX41 antibody (22-325). Western blot was performed from the immunoprecipitate using DDX41 antibody (22-325) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using DDX41 antibody (22-325) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DDX41 antibody (22-325). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DDX41 antibody (22-325). Western blot was performed from the immunoprecipitate using DDX41 antibody (22-325) at a dilition of 1:1000.

Further Information

Additional Names:
DDX41 Antibody: ABS, ABS, Probable ATP-dependent RNA helicase DDX41, DEAD box protein 41
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD box protein family and interacts with several spliceosomal proteins. In addition, the encoded protein may recognize the bacterial second messengers cyclic di-GMP and cyclic di-AMP, resulting in the induction of genes involved in the innate immune response.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).
NCBI Gene ID #:
51428
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
NCBI Official Symbol:
DDX41
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
Q9UJV9
User NOte:
Optimal dilutions for each application to be determined by the researcher.