FCN1 Antibody

ProSci
Product Code: PSI-22-334
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-334-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FCN1 antibody (22-334) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human tonsil using FCN1 Antibody (22-334) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human brain astrocytoma using FCN1 Antibody (22-334) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using FCN1 Antibody (22-334) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using FCN1 antibody (22-334) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using FCN1 Antibody (22-334) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain astrocytoma using FCN1 Antibody (22-334) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using FCN1 Antibody (22-334) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Ficolin-1, Collagen/fibrinogen domain-containing protein 1, Ficolin-A, Ficolin-alpha, M-ficolin, FCN1, FCNM
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-326 of human FCN1 (NP_001994.2).
NCBI Gene ID #:
2219
NCBI Official Name:
Ficolin-1
NCBI Official Symbol:
FCN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
O00602
User NOte:
Optimal dilutions for each application to be determined by the researcher.