FLOT2 Antibody

ProSci
Product Code: PSI-22-336
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-336-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FLOT2 antibody (22-336) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of MCF7 cells using FLOT2 antibody (22-336). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using FLOT2 antibody (22-336) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using FLOT2 antibody (22-336). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
FLOT2, flotillin 2, reggie-1, ESA, ESA1, M17S1, Flotillin 2 (epidermal surface antigen 1)
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 239-428 of human FLOT2 (NP_004466.2).
NCBI Gene ID #:
2319
NCBI Official Name:
flotillin 2
NCBI Official Symbol:
FLOT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q14254
User NOte:
Optimal dilutions for each application to be determined by the researcher.