GMPS Antibody

ProSci
Product Code: PSI-22-346
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-346-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using GMPS antibody (22-346) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GMPS antibody (22-346). Western blot was performed from the immunoprecipitate using GMPS antibody (22-346) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using GMPS antibody (22-346) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GMPS antibody (22-346). Western blot was performed from the immunoprecipitate using GMPS antibody (22-346) at a dilition of 1:1000.

Further Information

Additional Names:
GMPS,
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 394-693 of human GMPS (NP_003866.1).
NCBI Gene ID #:
8833
NCBI Official Name:
guanine monphosphate synthase
NCBI Official Symbol:
GMPS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P49915
User NOte:
Optimal dilutions for each application to be determined by the researcher.