GORASP1 Antibody

ProSci
Product Code: PSI-22-347
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-347-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GORASP1 antibody (22-347) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human gastric cancer using GORASP1 antibody (22-347) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using GORASP1 antibody (22-347). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GORASP1 antibody (22-347) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human gastric cancer using GORASP1 antibody (22-347) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using GORASP1 antibody (22-347). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Golgi reassembly-stacking protein 1, Golgi peripheral membrane protein p65, Golgi phosphoprotein 5, GOLPH5, Golgi reassembly-stacking protein of 65 kDa, GRASP65, GORASP1, GOLPH5, GRASP65
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GORASP1 (NP_114105.1).
NCBI Gene ID #:
64689
NCBI Official Name:
Golgi reassembly-stacking protein 1
NCBI Official Symbol:
GORASP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9BQQ3
User NOte:
Optimal dilutions for each application to be determined by the researcher.