Histone H2A.Z Antibody

ProSci
Product Code: PSI-22-352
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-352-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using H2AFZ antibody (22-352) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H2A.Z antibody (22-352) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using Histone H2A.Z antibody (22-352) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using Histone H2A.Z antibody (22-352) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using H2AFZ antibody (22-352) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H2A.Z antibody (22-352) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Histone H2A.Z antibody (22-352) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using Histone H2A.Z antibody (22-352) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Histone H2AZ, H2A/z, H2AFZ, H2AZ
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human H2AFZ (NP_002097.1).
NCBI Gene ID #:
3015
NCBI Official Name:
Histone H2A.Z
NCBI Official Symbol:
H2AFZ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 15kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P0C0S5
User NOte:
Optimal dilutions for each application to be determined by the researcher.