HOXB1 Antibody

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ProSci
Product Code: PSI-22-357
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-357-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse heart, using HOXB1 antibody (22-357) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of U-2 OS cells using HOXB1 antibody (22-357) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse heart, using HOXB1 antibody (22-357) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of U-2 OS cells using HOXB1 antibody (22-357) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
HCFP3, HOX2, HOX2I, Hox-2.9, homeobox protein Hox-B1, homeobox protein Hox-2I
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXB genes located in a cluster on chromosome 17.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 35-180 of human HOXB1 (NP_002135.2).
NCBI Gene ID #:
3211
NCBI Official Name:
homeobox B1
NCBI Official Symbol:
HOXB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
P14653
User NOte:
Optimal dilutions for each application to be determined by the researcher.