IL20 Antibody

ProSci
Product Code: PSI-22-363
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-363-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse liver, using IL20 Antibody (22-363) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver using IL20 antibody (22-363) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using IL20 antibody (22-363) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using IL20 antibody (22-363) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using IL20 antibody (22-363). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse liver, using IL20 Antibody (22-363) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using IL20 antibody (22-363) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL20 antibody (22-363) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using IL20 antibody (22-363) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using IL20 antibody (22-363). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
IL-20, IL10D, ZCYTO10, UNQ852/PRO1801, Interleukin-20, Cytokine Zcyto10, IL-20
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-176 of human IL20 (NP_061194.2).
NCBI Gene ID #:
50604
NCBI Official Name:
interleukin 20
NCBI Official Symbol:
IL20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Innate Immunity
Swissprot #:
Q9NYY1
User NOte:
Optimal dilutions for each application to be determined by the researcher.