KIF3A Antibody

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ProSci
Product Code: PSI-22-372
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-372-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using KIF3A antibody (22-372) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunofluorescence analysis of MCF7 cells using KIF3A antibody (22-372). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug KIF3A antibody (22-372). Western blot was performed from the immunoprecipitate using KIF3A antibody (22-372) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using KIF3A antibody (22-372) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using KIF3A antibody (22-372). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug KIF3A antibody (22-372). Western blot was performed from the immunoprecipitate using KIF3A antibody (22-372) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
KIF3A, FLA10, KLP-20
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3).
NCBI Gene ID #:
11127
NCBI Official Name:
kinesin family member 3A
NCBI Official Symbol:
KIF3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
Q9Y496
User NOte:
Optimal dilutions for each application to be determined by the researcher.