MMRN1 Antibody

ProSci
Product Code: PSI-22-384
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-384-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MMRN1 Antibody (22-384) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human lung using MMRN1 antibody (22-384) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using MMRN1 antibody (22-384). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MMRN1 Antibody (22-384) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human lung using MMRN1 antibody (22-384) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MMRN1 antibody (22-384). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Multimerin-1, EMILIN-4, Elastin microfibril interface located protein 4, Elastin microfibril interfacer 4, Endothelial cell multimerin, Platelet glycoprotein Ia*, 155 kDa platelet multimerin, p-155, p155, MMRN1, ECM, EMILIN4, GPIA*, MMRN
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 949-1228 of human MMRN1 (NP_031377.2).
NCBI Gene ID #:
22915
NCBI Official Name:
Multimerin-1
NCBI Official Symbol:
MMRN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 180kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13201
User NOte:
Optimal dilutions for each application to be determined by the researcher.