MYL3 Antibody

ProSci
Product Code: PSI-22-388
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-388-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MYL3 antibody (22-388) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 2
Immunofluorescence analysis of U2OS cells using MYL3 antibody (22-388). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MYL3 antibody (22-388) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of U2OS cells using MYL3 antibody (22-388). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MYL3, CMH8, Cardiac myosin light chain 1, CMLC1, Myosin, light chain 3, alkali, Myosin light chain 3, Ventricular, skeletal, slow, VLC1, MLC1SB, MLC1V
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human MYL3 (NP_000249.1).
NCBI Gene ID #:
4634
NCBI Official Name:
myosin, light chain 3, alkali; ventricular, skeletal, slow
NCBI Official Symbol:
MYL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 22kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P08590
User NOte:
Optimal dilutions for each application to be determined by the researcher.