NFATC3 Antibody

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ProSci
Product Code: PSI-22-392
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-392-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NFATC3 antibody (22-392) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunofluorescence analysis of HeLa cells using NFATC3 antibody (22-392). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using NFATC3 antibody (22-392) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of HeLa cells using NFATC3 antibody (22-392). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
NFATC3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3, NFAT4, NFATX, T cell transcription factor NFAT4, nuclear factor of activated T-cells c3 isoform IE-Xa, nuclear factor of activated T-cells, cytoplasmic 3
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human NFATC3 (NP_775188.1).
NCBI Gene ID #:
4775
NCBI Official Name:
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3?
NCBI Official Symbol:
NFATC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 150kDa
Purification:
Affinity purification
Research Area:
Immunology, Signal Transduction, Transcription
Swissprot #:
Q12968
User NOte:
Optimal dilutions for each application to be determined by the researcher.