NSFL1C Antibody

ProSci
Product Code: PSI-22-399
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-399-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using NSFL1C antibody (22-399) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 3
Immunofluorescence analysis of HeLa cells using NSFL1C antibody (22-399). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody (22-399). Western blot was performed from the immunoprecipitate using NSFL1C antibody (22-399) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using NSFL1C antibody (22-399) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using NSFL1C antibody (22-399). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody (22-399). Western blot was performed from the immunoprecipitate using NSFL1C antibody (22-399) at a dilition of 1:1000.

Further Information

Additional Names:
P47, UBX1, UBXD1UBXN2C, dJ776F14.1, NSFL1 cofactor p47, NSFL1 (p97) cofactor (p47), SHP1 homolog, UBX domain-containing protein 2C, p97 cofactor p47
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
NCBI Gene ID #:
55968
NCBI Official Name:
NSFL1 cofactor
NCBI Official Symbol:
NSFL1C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9UNZ2
User NOte:
Optimal dilutions for each application to be determined by the researcher.