NUDC Antibody

ProSci
Product Code: PSI-22-400
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-400-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NUDC antibody (22-400) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using NUDC antibody (22-400) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat stomach using NUDC antibody (22-400) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using NUDC antibody (22-400) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using NUDC antibody (22-400).

Western blot analysis of extracts of various cell lines, using NUDC antibody (22-400) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using NUDC antibody (22-400) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat stomach using NUDC antibody (22-400) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NUDC antibody (22-400) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using NUDC antibody (22-400).

Further Information

Additional Names:
NUDC, HNUDC, MNUDC, NPD011
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a nuclear distribution protein that plays an essential role in mitosis and cytokinesis. The encoded protein is involved in spindle formation during mitosis and in microtubule organization during cytokinesis. Pseudogenes of this gene are found on chromosome 2.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human NUDC (NP_006591.1).
NCBI Gene ID #:
10726
NCBI Official Name:
nudC nuclear distribution protein
NCBI Official Symbol:
NUDC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
Q9Y266
User NOte:
Optimal dilutions for each application to be determined by the researcher.