OGN Antibody

ProSci
Product Code: PSI-22-401
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-401-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using OGN antibody (22-401) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using OGN antibody (22-401) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using OGN antibody (22-401) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U-2 OS cells using OGN antibody (22-401) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using OGN antibody (22-401) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using OGN antibody (22-401) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using OGN antibody (22-401) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using OGN antibody (22-401) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Mimecan, Osteoglycin, Osteoinductive factor, OIF, OGN, OIF, SLRR3A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-298 of human OGN (NP_148935.1).
NCBI Gene ID #:
4969
NCBI Official Name:
Mimecan
NCBI Official Symbol:
OGN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P20774
User NOte:
Optimal dilutions for each application to be determined by the researcher.