PDCD6 Antibody

ProSci
Product Code: PSI-22-404
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-404-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PDCD6 antibody (22-404) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat kidney using PDCD6 antibody (22-404) at dilution of 1:200 (40x lens).
3 / 4
Immunofluorescence analysis of HeLa cells using PDCD6 antibody (22-404). Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PDCD6 antibody (22-404). Western blot was performed from the immunoprecipitate using PDCD6 antibody (22-404) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using PDCD6 antibody (22-404) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using PDCD6 antibody (22-404) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using PDCD6 antibody (22-404). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PDCD6 antibody (22-404). Western blot was performed from the immunoprecipitate using PDCD6 antibody (22-404) at a dilition of 1:1000.

Further Information

Additional Names:
Programmed cell death protein 6, Apoptosis-linked gene 2 protein, Probable calcium-binding protein ALG-2, PDCD6, ALG2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human PDCD6 (NP_037364.1).
NCBI Gene ID #:
10016
NCBI Official Name:
Programmed cell death protein 6
NCBI Official Symbol:
PDCD6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
O75340
User NOte:
Optimal dilutions for each application to be determined by the researcher.