PNKP Antibody

ProSci
Product Code: PSI-22-410
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-410-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PNKP antibody (22-410) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (22-410). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Western blot analysis of extracts of various cell lines, using PNKP antibody (22-410) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (22-410). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Further Information

Additional Names:
PNKP, polynucleotide kinase 3'-phosphatase, PNK, polynucleotide kinase 3-prime-phosphatase, MCSZ, EIEE10
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
NCBI Gene ID #:
11284
NCBI Official Name:
polynucleotide kinase 3'-phosphatase
NCBI Official Symbol:
PNKP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q96T60
User NOte:
Optimal dilutions for each application to be determined by the researcher.