PPP2CA Antibody

ProSci
Product Code: PSI-22-419
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-419-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PPP2CA antibody (22-419) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human stomach using PPP2CA antibody (22-419) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using PPP2CA antibody (22-419). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PPP2CA antibody (22-419) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using PPP2CA antibody (22-419) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using PPP2CA antibody (22-419). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PPP2CA, PP2Ac, PP2Calpha, ppp2A1, Protein phosphatase 2A alpha, Replication protein C, PP2CA, RP-C, PP2A-alpha
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PPP2CA (NP_002706.1).
NCBI Gene ID #:
5515
NCBI Official Name:
protein phosphatase 2, catalytic subunit, alpha isozyme
NCBI Official Symbol:
PPP2CA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P67775
User NOte:
Optimal dilutions for each application to be determined by the researcher.