PROK2 Antibody

ProSci
Product Code: PSI-22-421
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-421-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PROK2 antibody (22-421) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse spleen using PROK2 antibody (22-421) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using PROK2 antibody (22-421) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using PROK2 antibody (22-421) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using PROK2 antibody (22-421) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse spleen using PROK2 antibody (22-421) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PROK2 antibody (22-421) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PROK2 antibody (22-421) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Prokineticin 2, protein Bv8 homolog, PK2, MIT1, KAL4, BV8, prokineticin 2, PROK2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-129 of human PROK2 (NP_001119600.1).
NCBI Gene ID #:
60675
NCBI Official Name:
prokineticin 2
NCBI Official Symbol:
PROK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Growth Factors, Neuroscience
Swissprot #:
Q9HC23
User NOte:
Optimal dilutions for each application to be determined by the researcher.