RBP2 Antibody

ProSci
Product Code: PSI-22-433
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-433-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RBP2 antibody (22-433) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human liver damage using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using RBP2 antibody (22-433). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RBP2 antibody (22-433) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver damage using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RBP2 antibody (22-433). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Retinol-binding protein 2, Cellular retinol-binding protein II, CRBP-II, RBP2, CRBP2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes an abundant protein present in the small intestinal epithelium. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. This protein may also modulate the supply of retinoic acid to the nuclei of endometrial cells during the menstrual cycle.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human RBP2 (NP_004155.2).
NCBI Gene ID #:
5948
NCBI Official Name:
Retinol-binding protein 2
NCBI Official Symbol:
RBP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 15-17kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P50120
User NOte:
Optimal dilutions for each application to be determined by the researcher.