RPLP1 Antibody

ProSci
Product Code: PSI-22-439
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-439-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RPLP1 antibody (22-439) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human stomach using RPLP1 antibody (22-439) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using RPLP1 antibody (22-439) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RPLP1 antibody (22-439) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF7 cells using RPLP1 antibody (22-439). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RPLP1 antibody (22-439) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human stomach using RPLP1 antibody (22-439) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RPLP1 antibody (22-439) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPLP1 antibody (22-439) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using RPLP1 antibody (22-439). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
60S acidic ribosomal protein P1, RPLP1, RRP1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human RPLP1 (NP_000994.1).
NCBI Gene ID #:
6176
NCBI Official Name:
60S acidic ribosomal protein P1
NCBI Official Symbol:
RPLP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P05386
User NOte:
Optimal dilutions for each application to be determined by the researcher.