SPINT2 Antibody

ProSci
Product Code: PSI-22-459
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-459-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of MCF-7 cells, using SPINT2 Antibody (22-459) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunohistochemistry of paraffin-embedded human prostate using SPINT2 antibody (22-459) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using SPINT2 antibody (22-459). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of MCF-7 cells, using SPINT2 Antibody (22-459) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human prostate using SPINT2 antibody (22-459) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using SPINT2 antibody (22-459). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SPINT2, HAI-2, HAI2, Kop, PB, DIAR3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-197 of human SPINT2 (NP_066925.1).
NCBI Gene ID #:
10653
NCBI Official Name:
serine peptidase inhibitor, Kunitz type, 2
NCBI Official Symbol:
SPINT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
O43291
User NOte:
Optimal dilutions for each application to be determined by the researcher.