ST3GAL3 Antibody

ProSci
Product Code: PSI-22-462
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-462-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ST3GAL3 antibody (22-462) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver damage using ST3GAL3 antibody (22-462) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat lung using ST3GAL3 antibody (22-462) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using ST3GAL3 antibody (22-462) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using ST3GAL3 antibody (22-462). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ST3GAL3 antibody (22-462) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using ST3GAL3 antibody (22-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using ST3GAL3 antibody (22-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using ST3GAL3 antibody (22-462) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ST3GAL3 antibody (22-462). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ST3GAL3, SIAT6, ST3GALII, ST3GalIII, ST3N, MRT12, EIEE15
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Mutations in this gene have been associated with autosomal recessive nonsymdromic mental retardation-12 (MRT12). Multiple transcript variants encoding several different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-375 of human ST3GAL3 (NP_006270.1).
NCBI Gene ID #:
6487
NCBI Official Name:
ST3 beta-galactoside alpha-2,3-sialyltransferase 3
NCBI Official Symbol:
ST3GAL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 42kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q11203
User NOte:
Optimal dilutions for each application to be determined by the researcher.