TEAD1 Antibody

ProSci
Product Code: PSI-22-472
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-472-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TEAD1 antibody (22-472) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
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Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (22-472).
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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TEAD1 antibody (22-472) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (22-472) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (22-472).
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TEAD1 antibody (22-472) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Further Information

Additional Names:
TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEAD-1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

ChIP: 1:50 - 1:200
Background:
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
NCBI Gene ID #:
7003
NCBI Official Name:
TEA domain family member 1 (SV40 transcriptional enhancer factor)
NCBI Official Symbol:
TEAD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Transcription
Swissprot #:
P28347
User NOte:
Optimal dilutions for each application to be determined by the researcher.