TLK2 Antibody

ProSci
Product Code: PSI-22-473
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-473-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TLK2 antibody (22-473) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human liver damage using TLK2 antibody (22-473) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophageal cancer using TLK2 antibody (22-473) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using TLK2 antibody (22-473). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using TLK2 antibody (22-473) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using TLK2 antibody (22-473) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TLK2 antibody (22-473) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TLK2 antibody (22-473). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Serine/threonine-protein kinase tousled-like 2, HsHPK, PKU-alpha, Tousled-like kinase 2, TLK2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2).
NCBI Gene ID #:
11011
NCBI Official Name:
Serine/threonine-protein kinase tousled-like 2
NCBI Official Symbol:
TLK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 87kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
Q86UE8
User NOte:
Optimal dilutions for each application to be determined by the researcher.