TSC22D3 Antibody

ProSci
Product Code: PSI-22-479
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-479-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using TSC22D3 antibody (22-479) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse lung using TSC22D3 Antibody (22-479) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of U2OS cells using TSC22D3 antibody (22-479). Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TSC22D3 antibody (22-479). Western blot was performed from the immunoprecipitate using TSC22D3 antibody (22-479) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using TSC22D3 antibody (22-479) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using TSC22D3 Antibody (22-479) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TSC22D3 antibody (22-479). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TSC22D3 antibody (22-479). Western blot was performed from the immunoprecipitate using TSC22D3 antibody (22-479) at a dilition of 1:1000.

Further Information

Additional Names:
TSC22D3 Antibody: DIP, GILZ, hDIP, DSIPI, TSC-22R, TSC22 domain family protein 3, DSIP-immunoreactive peptide, Protein DIP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor κB. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human TSC22D3 (NP_001015881.1).
NCBI Gene ID #:
1831
NCBI Official Name:
TSC22 domain family, member 3
NCBI Official Symbol:
TSC22D3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 15kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
Q99576
User NOte:
Optimal dilutions for each application to be determined by the researcher.