TSHR Antibody

ProSci
Product Code: PSI-22-481
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-481-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using TSHR antibody (22-481) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using TSHR antibody (22-481) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
TSHR, LGR3, MGC75129, hTSHR-I, CHNG1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-253 of human TSHR (NP_001018046.1).
NCBI Gene ID #:
7253
NCBI Official Name:
thyroid stimulating hormone receptor
NCBI Official Symbol:
TSHR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Neuroscience, Signal Transduction
Swissprot #:
P16473
User NOte:
Optimal dilutions for each application to be determined by the researcher.