UQCRFS1 Antibody

ProSci
Product Code: PSI-22-484
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-484-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using UQCRFS1 antibody (22-484) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of U2OS cells using UQCRFS1 antibody (22-484). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using UQCRFS1 antibody (22-484) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using UQCRFS1 antibody (22-484). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Cytochrome b-c1 complex subunit Rieske, mitochondrial, Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, RISP, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Cytochrome b-c1 complex subunit 11, Complex III subunit IX, Ubiquinol-cytochrome c reductase 8 kDa protein, UQCRFS1
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 79-274 of human UQCRFS1 (NP_005994.2).
NCBI Gene ID #:
7386
NCBI Official Name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial
NCBI Official Symbol:
UQCRFS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P47985
User NOte:
Optimal dilutions for each application to be determined by the researcher.