Bcl9 Antibody

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ProSci
Product Code: PSI-22-491
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-491-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Bcl9 antibody (22-491) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using BCL9 antibody (22-491) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using BCL9 antibody (22-491) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using Bcl9 antibody (22-491) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using BCL9 antibody (22-491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using BCL9 antibody (22-491) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
2610202E01Rik, 8030475K17Rik, A330041G23Rik, Gm13B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, bcl-9, nuclear co-factor of beta-catenin signalling
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of mouse Bcl9
NCBI Gene ID #:
77578
NCBI Official Name:
B cell CLL/lymphoma 9
NCBI Official Symbol:
BCL9
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 160kDa/170kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Stem Cell, Transcription
User NOte:
Optimal dilutions for each application to be determined by the researcher.