POLR2G Antibody

ProSci
Product Code: PSI-22-504
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-504-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using POLR2G antibody (22-504) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using POLR2G antibody (22-504) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Further Information

Additional Names:
RNA polymerase II seventh subunit, DNA directed RNA polymerase II polypeptide G, DNA directed RNA polymerase II 19 kda polypeptide, hsRPB7, hRPB19, RPB7, MGC138369, MGC138367, polymerase (RNA) II (DNA directed) polypeptide G, POLR2G
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human POLR2G (NP_002687.1).
NCBI Gene ID #:
5436
NCBI Official Name:
polymerase (RNA) II (DNA directed) polypeptide G
NCBI Official Symbol:
POLR2G
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P62487
User NOte:
Optimal dilutions for each application to be determined by the researcher.