WDR48 Antibody

ProSci
Product Code: PSI-22-509
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-509-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using WDR48 antibody (22-509) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 4
Immunohistochemistry of paraffin-embedded rat liver using WDR48 antibody (22-509) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human liver using WDR48 antibody (22-509) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human stomach using WDR48 antibody (22-509) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using WDR48 antibody (22-509) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat liver using WDR48 antibody (22-509) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using WDR48 antibody (22-509) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using WDR48 antibody (22-509) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
WD repeat-containing protein 48, USP1-associated factor 1, WD repeat endosomal protein, p80, WDR48, KIAA1449, UAF1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 450-677 of human WDR48 (NP_065890.1).
NCBI Gene ID #:
57599
NCBI Official Name:
WD repeat-containing protein 48
NCBI Official Symbol:
WDR48
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 76kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q8TAF3
User NOte:
Optimal dilutions for each application to be determined by the researcher.