DGAT1 Antibody

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ProSci
Product Code: PSI-22-510
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-510-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DGAT1 antibody (22-510) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using DGAT1 antibody (22-510) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

Documents

Further Information

Additional Names:
DGAT1, diacylglycerol O-acyltransferase 1, ARGP1, DGAT, ACAT related gene product 1, ACAT-related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase homolog 1, diglyceride acyltransferase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human DGAT1 (NP_036211.2).
NCBI Gene ID #:
8694
NCBI Official Name:
diacylglycerol O-acyltransferase 1
NCBI Official Symbol:
DGAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
O75907
User NOte:
Optimal dilutions for each application to be determined by the researcher.