ATOX1 Antibody

ProSci
Product Code: PSI-22-522
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-522-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATOX1 Antibody (22-522) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 2
Immunofluorescence analysis of MCF7 cells using ATOX1 antibody (22-522). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ATOX1 Antibody (22-522) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of MCF7 cells using ATOX1 antibody (22-522). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ATX1, HAH1, copper transport protein ATOX1, ATX1 antioxidant protein 1 homolog, metal transport protein ATX1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human ATOX1 (NP_004036.1).
NCBI Gene ID #:
475
NCBI Official Name:
antioxidant 1 copper chaperone
NCBI Official Symbol:
ATOX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O00244
User NOte:
Optimal dilutions for each application to be determined by the researcher.