C1S Antibody

ProSci
Product Code: PSI-22-525
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-525-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HepG2 cells, using C1S antibody (22-525) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using C1S antibody (22-525) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using C1S antibody (22-525) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of A-549 cells using C1S antibody (22-525).

Western blot analysis of extracts of HepG2 cells, using C1S antibody (22-525) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using C1S antibody (22-525) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using C1S antibody (22-525) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A-549 cells using C1S antibody (22-525).

Further Information

Additional Names:
EDSPD2, complement C1s subcomponent, C1 esterase, basic proline-rich peptide IB-1, complement component 1 subcomponent s, complement component 1, s subcomponent
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 479-688 of human C1S (NP_958850.1).
NCBI Gene ID #:
716
NCBI Official Name:
complement C1s
NCBI Official Symbol:
C1S
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 77kDa
Purification:
Affinity purification
Research Area:
Immunology, Innate Immunity, Signal Transduction
Swissprot #:
P09871
User NOte:
Optimal dilutions for each application to be determined by the researcher.