SLC25A20 Antibody

ProSci
Product Code: PSI-22-526
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-526-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SLC25A20 antibody (22-526) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of HeLa cells using SLC25A20 antibody (22-526). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SLC25A20 antibody (22-526) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using SLC25A20 antibody (22-526). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SLC25A20, CAC, CACT
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene product is one of several closely related mitochondrial-membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. This protein mediates the transport of acylcarnitines into mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway. Mutations in this gene are associated with carnitine-acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human SLC25A20 (NP_000378.1).
NCBI Gene ID #:
788
NCBI Official Name:
solute carrier family 25 (carnitine/acylcarnitine translocase), member 20
NCBI Official Symbol:
SLC25A20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 33kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O43772
User NOte:
Optimal dilutions for each application to be determined by the researcher.