SEPT7 Antibody

ProSci
Product Code: PSI-22-529
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-529-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SEPT7 antibody (22-529) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using SEPT7 antibody (22-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse Intestine using SEPT7 antibody (22-529) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using SEPT7 antibody (22-529) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using SEPT7 antibody (22-529).

Western blot analysis of extracts of various cell lines, using SEPT7 antibody (22-529) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human lung cancer using SEPT7 antibody (22-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse Intestine using SEPT7 antibody (22-529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SEPT7 antibody (22-529) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using SEPT7 antibody (22-529).

Further Information

Additional Names:
SEPT7, septin 7, CDC10, CDC3, Nbla02942, SEPT7A, CDC10 (cell division cycle 10, S. cerevisiae, homolog), CDC10 cell division cycle 10 homolog, cell division cycle 10
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 307-436 of human SEPT7 (NP_001011553.2).
NCBI Gene ID #:
989
NCBI Official Name:
septin 7
NCBI Official Symbol:
SEPT7.
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Chemokines & Cytokines, Immunology
Swissprot #:
Q16181
User NOte:
Optimal dilutions for each application to be determined by the researcher.