GABPB1 Antibody

ProSci
Product Code: PSI-22-547
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-547-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GABPB1 antibody (22-547) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse kidney using GABPB1 Antibody (22-547) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using GABPB1 Antibody (22-547) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using GABPB1 Antibody (22-547) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF7 cells using GABPB1 antibody (22-547).

Western blot analysis of extracts of various cell lines, using GABPB1 antibody (22-547) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse kidney using GABPB1 Antibody (22-547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GABPB1 Antibody (22-547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GABPB1 Antibody (22-547) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using GABPB1 antibody (22-547).

Further Information

Additional Names:
GABPB2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GABPB2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 231-395 of human GABPB1 (NP_005245.2).
NCBI Gene ID #:
2553
NCBI Official Name:
GA binding protein transcription factor, beta subunit 1
NCBI Official Symbol:
GABPB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q06547
User NOte:
Optimal dilutions for each application to be determined by the researcher.