MSTN Antibody

ProSci
Product Code: PSI-22-550
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-550-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MSTN antibody (22-550) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunohistochemistry of paraffin-embedded mouse lung using MSTN antibody (22-550) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using MSTN antibody (22-550) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using MSTN antibody (22-550) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
MSTN, GDF8, MSLHP, Myostatin, GDF-8
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 267-375 of human MSTN (NP_005250.1).
NCBI Gene ID #:
2660
NCBI Official Name:
myostatin
NCBI Official Symbol:
MSTN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 43kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Signal Transduction
Swissprot #:
O14793
User NOte:
Optimal dilutions for each application to be determined by the researcher.