GOT2 Antibody

ProSci
Product Code: PSI-22-551
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-551-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GOT2 antibody (22-551) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat kidney using GOT2 antibody (22-551) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using GOT2 antibody (22-551) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat kidney using GOT2 antibody (22-551) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
GOT2, FLJ40994, kat4, kativ, mitaat, KAT4, KATIV, mitAAT
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human GOT2 (NP_002071.2).
NCBI Gene ID #:
2806
NCBI Official Name:
glutamic-oxaloacetic transaminase 2, mitochondrial
NCBI Official Symbol:
GOT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P00505
User NOte:
Optimal dilutions for each application to be determined by the researcher.