GPS1 Antibody

ProSci
Product Code: PSI-22-553
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-553-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunofluorescence analysis of MCF7 cells using GPS1 antibody (22-553).
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Immunohistochemistry of paraffin-embedded mouse cancer using GPS1 antibody (22-553) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using GPS1 antibody (22-553) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using GPS1 antibody (22-553) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using GPS1 antibody (22-553) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

Immunofluorescence analysis of MCF7 cells using GPS1 antibody (22-553).
Immunohistochemistry of paraffin-embedded mouse cancer using GPS1 antibody (22-553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GPS1 antibody (22-553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using GPS1 antibody (22-553) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using GPS1 antibody (22-553) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
GPS1, G protein pathway suppressor 1, COPS1, Arabidopsis FUS6/COP11 homolog, CSN1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene is known to suppress G-protein and mitogen-activated signal transduction in mammalian cells. The encoded protein shares significant similarity with Arabidopsis FUS6, which is a regulator of light-mediated signal transduction in plant cells.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human GPS1 (NP_997657.1).
NCBI Gene ID #:
2873
NCBI Official Name:
G protein pathway suppressor 1
NCBI Official Symbol:
GPS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q13098
User NOte:
Optimal dilutions for each application to be determined by the researcher.