LGALS3BP Antibody

ProSci
Product Code: PSI-22-563
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-563-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using LGALS3BP antibody (22-563) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of MCF7 cells using LGALS3BP antibody (22-563). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using LGALS3BP antibody (22-563) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using LGALS3BP antibody (22-563). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
LGALS3BP, 90K, MAC-2-BP, BTBD17B, TANGO10B
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 306-585 of human LGALS3BP (NP_005558.1).
NCBI Gene ID #:
3959
NCBI Official Name:
lectin, galactoside-binding, soluble, 3 binding protein
NCBI Official Symbol:
LGALS3BP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa-110kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
Q08380
User NOte:
Optimal dilutions for each application to be determined by the researcher.