MYH1 Antibody

ProSci
Product Code: PSI-22-566
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-566-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MYH1 antibody (22-566) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat brain using MYH1 Antibody (22-566) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using MYH1 antibody (22-566). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MYH1 antibody (22-566) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using MYH1 Antibody (22-566) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MYH1 antibody (22-566). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HEL71, MYHSA1, MYHa, MyHC-2X/D, MyHC-2x, myosin-1, epididymis luminal protein 71, myHC-IIx/d, myosin heavy chain 2x, myosin heavy chain IIx/d, myosin heavy chain, skeletal muscle, adult 1, myosin, heavy chain 1, skeletal muscle, adult, myosin, heavy polypeptide 1, skeletal muscle, adult
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MYH1 (NP_005954.3).
NCBI Gene ID #:
4619
NCBI Official Name:
myosin heavy chain 1
NCBI Official Symbol:
MYH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 260kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P12882
User NOte:
Optimal dilutions for each application to be determined by the researcher.