MYO1C Antibody

ProSci
Product Code: PSI-22-567
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-567-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MYO1C antibody (22-567) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF7 cells using MYO1C antibody (22-567).

Western blot analysis of extracts of various cell lines, using MYO1C antibody (22-567) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using MYO1C antibody (22-567).

Further Information

Additional Names:
Unconventional myosin-Ic, Myosin I beta, MMI-beta, MMIb, MYO1C
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 769-1028 of human MYO1C (NP_203693.3).
NCBI Gene ID #:
4641
NCBI Official Name:
Unconventional myosin-Ic
NCBI Official Symbol:
MYO1C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 122kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O00159
User NOte:
Optimal dilutions for each application to be determined by the researcher.