POLR2H Antibody

ProSci
Product Code: PSI-22-578
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-578-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using POLR2H antibody (22-578) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug POLR2H antibody (22-578). Western blot was performed from the immunoprecipitate using POLR2H antibody (22-578) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using POLR2H antibody (22-578) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug POLR2H antibody (22-578). Western blot was performed from the immunoprecipitate using POLR2H antibody (22-578) at a dilition of 1:1000.

Further Information

Additional Names:
POLR2H, RPABC3, RPB17, RPB8, hsRPB8
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:200
Background:
The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).
NCBI Gene ID #:
5437
NCBI Official Name:
polymerase (RNA) II (DNA directed) polypeptide H
NCBI Official Symbol:
POLR2H
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P52434
User NOte:
Optimal dilutions for each application to be determined by the researcher.