PPID Antibody

ProSci
Product Code: PSI-22-579
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-579-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PPID antibody (22-579) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using PPID antibody (22-579). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PPID antibody (22-579) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using PPID antibody (22-579). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
peptidylprolyl isomerase D (cyclophilin D), CYP-40, MGC33096, 40 kDa peptidyl-prolyl cis-trans isomerase D, PPIase, cyclophilin 40, cyclophilin D, cyclophilin-related protein, peptidylprolyl isomerase D, rotamase, CYP40, CYPD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 161-370 of human PPID (NP_005029.1).
NCBI Gene ID #:
5481
NCBI Official Name:
peptidylprolyl isomerase D
NCBI Official Symbol:
PPID
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Immunology, Neuroscience
Swissprot #:
Q08752
User NOte:
Optimal dilutions for each application to be determined by the researcher.