SRGN Antibody

ProSci
Product Code: PSI-22-581
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-581-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of THP-1 cells, using SRGN antibody (22-581) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human liver damage using SRGN antibody (22-581) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using SRGN antibody (22-581). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of THP-1 cells, using SRGN antibody (22-581) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using SRGN antibody (22-581) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using SRGN antibody (22-581). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Serglycin, Hematopoietic proteoglycan core protein, Platelet proteoglycan core protein, PPG, Secretory granule proteoglycan core protein, SRGN, PRG, PRG1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This encoded protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-158 of human SRGN (NP_002718.2).
NCBI Gene ID #:
5552
NCBI Official Name:
Serglycin
NCBI Official Symbol:
SRGN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Obesity, Signal Transduction
Swissprot #:
P10124
User NOte:
Optimal dilutions for each application to be determined by the researcher.